Insertion junction: CLIP2.002072_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.002072
Chromosome:chromosome 17
Location:3304215
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g722300 (1 of 26) IPR017850 - Alkaline-phosphatase-like, core domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCAGCGTAAGAGCCATATACGCCATAGAAGTTGCCATAGTATCCGTAC
Internal bar code:AGGCGTGTTTGCGGTAGCTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:4235
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:60
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:AAATTGCTTGTTCACGCGCT
Suggested primer 2:AATGCAAGAAAACCGCCGAC