| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.002080 |
| Chromosome: | chromosome 1 |
| Location: | 6110463 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g043350 | CAO1,CAO | Chlorophyllide a oxygenase, chloroplast precursor; (1 of 1) K13600 - chlorophyllide a oxygenase (CAO) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGCGTATGGGGGCGTCCAGGCCCGCGTGCATCGGGCGCACGCAATCT |
| Internal bar code: | CGCGTCGTATCGAGCTAACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1316 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGACCTCTGTAAGATCGCC |
| Suggested primer 2: | AAAATGCAGATGCGGCGATC |