Insertion junction: CLIP2.002080_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.002080
Chromosome:chromosome 1
Location:6110474
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g043350 CAO1,CAO Chlorophyllide a oxygenase, chloroplast precursor; (1 of 1) K13600 - chlorophyllide a oxygenase (CAO) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATACGCCAGCGTCCGCCGCAATACATCATACAACGTCCAGTTGTAGGGC
Internal bar code:CGCGTCGTATCGAGCTAACTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1754
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:60
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:AAAATGCAGATGCGGCGATC
Suggested primer 2:CCGACCTCTGTAAGATCGCC