Insertion junction: CLIP2.002101_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.002101
Chromosome:chromosome 6
Location:4749555
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g279500 CPL6 Conserved in the Plant Lineage; (1 of 1) PTHR15852:SF8 - CHAPERONE PROTEIN DNAJ-LIKE PROTEIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCTGAACGCAGCGTCCTCGGAGCTGCCGTCGTCCGCGTCCGCGGCGAA
Internal bar code:GTGTATTCTCTGACGAGAGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:3493
LEAP-Seq percent confirming:9.23077
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:59
LEAP-Seq n unique pos:65

Suggested primers for confirmation by PCR

Suggested primer 1:CCTAGCTTCCTTCTCGAGCG
Suggested primer 2:CGCGGTTTGATCAGCAACAA