Insertion junction: CLIP2.002105_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.002105
Chromosome:chromosome 6
Location:7427133
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g300500 ADCY1,CYA18 Adenylyl cyclase; (1 of 1) PTHR11347//PTHR11347:SF132 - CYCLIC NUCLEOTIDE PHOSPHODIESTERASE // PDE1C, ISOFORM E intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCTGTGGCGCCTGCACCTGCTGTCCTTGCAACACGTCCGCGCCGCGCG
Internal bar code:CCGGAGAACGGATTTCAACAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:749
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AACGATGGGTAAGCAGCACA
Suggested primer 2:GCCTCCTGTGTTCCTCCTTC