| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.002139 |
| Chromosome: | chromosome 10 |
| Location: | 1726763 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430100 | ELG22 | Exostosin-like glycosyltransferase 22; (1 of 6) IPR000742//IPR004263//IPR009030 - EGF-like domain // Exostosin-like // Insulin-like growth factor binding protein, N-terminal | 5'UTR |
| Cre10.g430150 | REP27,LPA1 | (1 of 1) PF11998//PF13414 - Protein of unknown function (DUF3493) (DUF3493) // TPR repeat (TPR_11); Low Photosystem II Accumulation 1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAAAGGGTCAGAAAGTGAATTGGTAAAATTAGAAGCGCTGCCCCATG |
| Internal bar code: | GAAATGTGTTAGCTCATAAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1757 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATTTCCAAGCCAACACG |
| Suggested primer 2: | ATGGAGCAGTCAAGACCGTG |