Insertion junction: CLIP2.002144_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.002144
Chromosome:chromosome 12
Location:4756077
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g523050 CCT11,GEX204 (1 of 1) 2.7.1.150 - 1-phosphatidylinositol-3-phosphate 5-kinase / Type III PIP kinase; Putative T-complex protein 1 component CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCATGACCATGAACTCGCCCTTTGCGGCGCAGGCGGGCTCGCGCTGGGC
Internal bar code:CACTTATTACATTCCGTCCACC

Confirmation - LEAP-Seq

LEAP-Seq distance:1024
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTTTCCCTTCCATTCCCT
Suggested primer 2:CCGGCATCTACCTGTCCATC