Insertion junction: CLIP2.002162_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.002162
Chromosome:chromosome 9
Location:6511776
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g415650 FAP351 Flagellar Associated Protein 351; (1 of 7) PF07004 - Sperm-tail PG-rich repeat (SHIPPO-rpt) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGACCAGAACTCAAACAAGTCATCCGCCTGCACCCCTGCTTCCACTCA
Internal bar code:TGAAGAAGGTGGGGGACTCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1672
LEAP-Seq percent confirming:87.0968
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:ACGAAAAACAAGCAGCGCAT
Suggested primer 2:TCGCACTGCTGACATGACTT