| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.002164 |
| Chromosome: | chromosome 16 |
| Location: | 2422572 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659850 | CGL37 | conserved protein related to shikimate kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGGAAATGCAAAGGACATGTGTGCGGGTGTGTGGGTGTGAGCGGGTA |
| Internal bar code: | ATTCCCCTGACTTGGCCTAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 191 |
| LEAP-Seq percent confirming: | 27.7778 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATTGGTGGGTGTCAGAGG |
| Suggested primer 2: | TAGGGTGAGCAAAAGCAGCA |