Insertion junction: CLIP2.002165_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.002165
Chromosome:chromosome 13
Location:1105295
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g569250 CGLD9 Conserved in the Green Lineage and Diatoms; (1 of 1) PF11255 - Protein of unknown function (DUF3054) (DUF3054) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACTCATCTCCTAGTGGCTCCCGACACCCCTCTTACATAACCCGACAGCC
Internal bar code:CGGGATCTTGGATCGTGGAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2296
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CCACTTGCTGGGCAACTTTC
Suggested primer 2:GAACTCTCAACCTCCAGCCC