Insertion junction: CLIP2.002169_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.002169
Chromosome:chromosome 1
Location:6286228
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g044650 (1 of 1) PF00168//PF17047 - C2 domain (C2) // Synaptotagmin-like mitochondrial-lipid-binding domain (SMP_LBD) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGCCAAAACCTGCTATCAGGACCCTGTCTCTATTAACAATGGCGGGCC
Internal bar code:GGGGTTCACTGGACGACACTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:580
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TCCACCAGCATCCAACCTTC
Suggested primer 2:GGCTTTGCGCTTCTTGTTCA