| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.002173 |
| Chromosome: | chromosome 3 |
| Location: | 3402429 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g166700 | FOR1 | (1 of 3) PTHR23213//PTHR23213:SF214 - FORMIN-RELATED // SUBFAMILY NOT NAMED; Formin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGACCCGAGCTTTAGCCCACCTGACGTGTTGTTGACCGCGTGCCCCA |
| Internal bar code: | TGCCCATCGGATTGCTGCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4186 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAATCCTTGCTTGCCGATG |
| Suggested primer 2: | TACTGGTGCTGCGAGTCAAG |