Insertion junction: CLIP2.002174_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.002174
Chromosome:chromosome 13
Location:4428092
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g602400 KIN8A,KIN8-1 Kinesin motor protein; (1 of 1) K10401 - kinesin family member 18/19 (KIF18_19) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAGGACGCCAACCTGTACAACAAGCACGAGCTCAGCCAGCTGGACGAGT
Internal bar code:TGGTGGCTTGGATCATTTGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:4664
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTGGACAGCAGGTTGAGG
Suggested primer 2:GTAAATGCGGAATGGCGGTC