Insertion junction: CLIP2.002211_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.002211
Chromosome:chromosome 6
Location:1609836
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g261500 CPLD58 Conserved in the Plant Lineage and Diatoms; (1 of 1) PTHR11260:SF136 - THIOREDOXIN FAMILY PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCCACTGCTTCTGGTTCTGCTATACGGTGTCCTAACTACTGCGGCAGT
Internal bar code:GTGGGGTTAATACGATTCGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:2229
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:93
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:93

Suggested primers for confirmation by PCR

Suggested primer 1:GTAACCACACACGCACACAC
Suggested primer 2:TGAGCACACGTGTCTCTCAC