Insertion junction: CLIP2.002219_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.002219
Chromosome:chromosome 3
Location:635929
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145587 CPLD1 Conserved in the Plant Lineage and Diatoms; (1 of 4) PF04134 - Protein of unknown function, DUF393 (DUF393) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCTGATTCCTGCGTTCTCCGCGGGGTTGTAGTCGGCGGCAGCAATGTCC
Internal bar code:TGCCAGGTGGGCTCATTCCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1890
LEAP-Seq percent confirming:86.3636
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:CATGTTGGTGTGGTGCTGTG
Suggested primer 2:TTTGATGCCCACACGTCGTA