| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.002223 |
| Chromosome: | chromosome 9 |
| Location: | 2550044 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392700 | CGL39 | Conserved in the Green Lineage | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACGCCAGCTCCGCCTCCAGCGCCCGGAAGGACGCGGGCGGTGGCGGCG |
| Internal bar code: | CATGGTTATGCCATACGATAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1152 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCAGAATTCGAAGCGTGA |
| Suggested primer 2: | CCGGTACAGAATCGTGCACT |