| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.002229 |
| Chromosome: | chromosome 2 |
| Location: | 113241 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g073900 | (1 of 2) 1.13.11.69 - Carlactone synthase | 3'UTR | |
| Cre02.g074000 | POC18 | Proteome of centriole protein 18; (1 of 1) PTHR19853//PTHR19853:SF1 - WD REPEAT CONTAINING PROTEIN 3 WDR3 // TBC1 DOMAIN FAMILY MEMBER 31 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTTCGTCAGGACGAGCCCGTACCAGGGAGCTGGCCATCCAACGCACTG |
| Internal bar code: | TTTAAAAATTGTTATCCGTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2826 |
| LEAP-Seq percent confirming: | 97.2222 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCTTCCCAGCAGGTGAG |
| Suggested primer 2: | CCTACCGCTACTGCTACAGC |