Insertion junction: CLIP2.002236_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.002236
Chromosome:chromosome 9
Location:1523041
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g399400 TGL15,FAP199 Lipase-Domain Containing Flagellar Associated Protein 199; (1 of 22) PF01764 - Lipase (class 3) (Lipase_3) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACCCCTCGTTGGCATACGGCATCAGCTCCAGCGCCACCTACTGCGTGA
Internal bar code:GTACATGTGCCGATTTAGAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2293
LEAP-Seq percent confirming:54.5455
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCAAGCAAGGGTAAGGTA
Suggested primer 2:TCCATAGCTAGCTCTTGCGC