Insertion junction: CLIP2.002268_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.002268
Chromosome:chromosome 13
Location:1962212
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g576400 ADCL1,BCA4 aminotransferase related to 4-amino-4-deoxychorismate lyase; (1 of 1) 2.6.1.21//4.1.3.38 - D-amino-acid transaminase / D-aspartic aminotransferase // Aminodeoxychorismate lyase / Enzyme X intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCAGGTTGGACGCATGGCGCCGAGTGAATGATGGTTGAGGGCTTGCGT
Internal bar code:ATAGGGTCAGAGGGATTTAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:4214
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:95
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:95

Suggested primers for confirmation by PCR

Suggested primer 1:GGACTAGAGGGCGCTTTGTT
Suggested primer 2:GCTATTCACGGACGAGGAGG