Insertion junction: CLIP2.002275_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.002275
Chromosome:chromosome 7
Location:831089
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g318350 CGL64 (1 of 4) PF12576 - Protein of unknown function (DUF3754) (DUF3754); Conserved in the Green Lineage CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTTCTTGATGACGGCCCAGGCGGTGGGCATGTGCAGCCGCAGCGTCTTG
Internal bar code:ACATTCGGTGATGAAAAAAAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:346
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GACACACACACGCTCTCTCA
Suggested primer 2:CTTCATGTGGATGTTGCGCC