Insertion junction: CLIP2.002301_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.002301
Chromosome:chromosome 2
Location:2267062
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g090100 (1 of 1) PTHR22883//PTHR22883:SF23 - ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN // PALMITOYLTRANSFERASE ZDHHC4-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACGCACTCTTACAGCCCTTGTGTGTACTACCCGCCATGATGCTTTCA
Internal bar code:ACTTGTACTGGGGCGAACAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:165
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTACCTATCACGCACACC
Suggested primer 2:GCCTCCCTGACTCACTCCTA