| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.002317 |
| Chromosome: | chromosome 16 |
| Location: | 5525839 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682200 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAGGGCCCTAAACGTCGCCACCGCTGCGGTATCCTCCCTCGCTGCGGT |
| Internal bar code: | GAGTTTGGTCGGGAAAGCATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 295 |
| LEAP-Seq percent confirming: | 57.1429 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTCTTCACGGAGGTTCAC |
| Suggested primer 2: | TCGGTGTCAGAACTTGTCGG |