Insertion junction: CLIP2.002337_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.002337
Chromosome:chromosome 1
Location:6171803
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g043850 (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGTACTGGTGCTGCCAATGCTGCTGGTGGTGCTAGTGCTTGCGTTCGA
Internal bar code:ATATCAAGGGATCAACTTATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:5272
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:130
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:130

Suggested primers for confirmation by PCR

Suggested primer 1:TGTATGGCCGAACGGTTTCA
Suggested primer 2:ATCACAATCAGGCTGCAGCT