Insertion junction: CLIP2.002366_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.002366
Chromosome:chromosome 3
Location:8720577
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g208721 CGL92 (1 of 2) 2.7.6.5 - GTP diphosphokinase / Stringent factor; predicted protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCCGCTGCTGCTGCCGGCGCAAGCACTGCTACTGCCGACAACAACACC
Internal bar code:GTATCGACCGGCCATGGGACGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1668
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGAAAGCGAGACCTTGTGGA
Suggested primer 2:CTCTCAGCACCGACACAAGT