| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.002372 |
| Chromosome: | chromosome 10 |
| Location: | 5503182 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457900 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAGCAGAAGCACGCCAAACGTCGTCTACGCAACGGTCGAAAACGTGGA |
| Internal bar code: | TTACAAGTAATTAATATGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 841 |
| LEAP-Seq percent confirming: | 58.3333 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGATCAAACGTCGCAATT |
| Suggested primer 2: | TCACAAATGGCCTTGCATGC |