| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.002403 |
| Chromosome: | chromosome 3 |
| Location: | 4412231 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175100 | (1 of 1) IPR013750//IPR016035 - GHMP kinase, C-terminal domain // Acyl transferase/acyl hydrolase/lysophospholipase | 3'UTR | |
| Cre03.g175200 | TOC75 | Translocon at the outer envelope membrane of chloroplasts, 75 kDa subunit; (1 of 2) PTHR12815//PTHR12815:SF7 - SORTING AND ASSEMBLY MACHINERY SAM50 PROTEIN // OUTER ENVELOPE PROTEIN 80, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAAAGTGCTTGTTCATTCTCACATCGGCGAGCGGCAGCGTCTGCAGG |
| Internal bar code: | CGGAAACCACAACAGTGGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5158 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCGAGAATACCCATGGC |
| Suggested primer 2: | ATAGGATGAGGTGGGGTGCT |