| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.002413 |
| Chromosome: | chromosome 10 |
| Location: | 1660978 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429601 | (1 of 2) PTHR10060:SF22 - CELL DEATH-RELATED NUCLEASE 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTAGCATGCGTGTATGATGGAACTTGATGCACGGAATGACATGTACTG |
| Internal bar code: | GATGGCTGTGTCTGGCCCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 267 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCTTGTGCCGTCTGACAC |
| Suggested primer 2: | TGACCACGTGACCAATGAGG |