Insertion junction: CLIP2.002457_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.002457
Chromosome:chromosome 2
Location:2423977
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g091400 CLPB2 (1 of 1) IPR001270//IPR003593//IPR003959//IPR024064//IPR027417 - ClpA/B family // AAA+ ATPase domain // ATPase, AAA-type, core // FdhE-like // P-loop containing nucleoside triphosphate hydrolase; ClpB chaperone, Hsp100 family CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCTTCATGGATGTCGTCAAGGGTAAGGTCGTGCGCAACGTGTGTGGGG
Internal bar code:GGTGCTGTTAGGGACATCGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1449
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:GCTATAAACCTGCGCAACCG
Suggested primer 2:CCCCTGCTGTGTCACATCAT