| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.002460 |
| Chromosome: | chromosome 13 |
| Location: | 2424351 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579450 | CST1 | Chlamydomonas-specific membrane transporter of unknown function | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCACACTTGTCCTGGACAGAGGCAAGGCCCGCCAGCGACACAGCCCTG |
| Internal bar code: | CTGACCCGTGGGTCACTGTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2285 |
| LEAP-Seq percent confirming: | 94.8276 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGACCTTCCGAAAGACGA |
| Suggested primer 2: | GGCCGAGTCACAGCTTTAGT |