| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.002484 |
| Chromosome: | chromosome 3 |
| Location: | 546455 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145187 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | 5'UTR | |
| Cre03.g145207 | CPLD33 | Conserved in the Plant Lineage and Diatoms; (1 of 1) PTHR36359:SF1 - RESISTANCE TO PHYTOPHTHORA 1 PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTTTCTGATACCAACTCAAAAGCCCTGCTTTTCCTGGAACCTTACTCG |
| Internal bar code: | GTCGTACTTGAGCTAATTGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2176 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCTTCGTACTCGGAAGC |
| Suggested primer 2: | CGTGCCGACTCAGATAGGTC |