Insertion junction: CLIP2.002484_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.002484
Chromosome:chromosome 3
Location:546466
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145187 (1 of 274) IPR020683 - Ankyrin repeat-containing domain 5'UTR
Cre03.g145207 CPLD33 Conserved in the Plant Lineage and Diatoms; (1 of 1) PTHR36359:SF1 - RESISTANCE TO PHYTOPHTHORA 1 PROTEIN 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCGTCCCAAAACTCAATAGTGTTGCATGCCGTTGTAACGATGTGTTGAA
Internal bar code:GTCGTACTTGAGCTAATTGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1381
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGCCGACTCAGATAGGTC
Suggested primer 2:CCCTCTTCGTACTCGGAAGC