| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.002486 |
| Chromosome: | chromosome 12 |
| Location: | 6978576 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g561000 | CYN28 | (1 of 1) PTHR11071//PTHR11071:SF286 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // SUBFAMILY NOT NAMED; Cyclophilin 28 | 3'UTR |
| Cre12.g561050 | ELG18,ELG19 | (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase; Exostosin-like glycosyltransferase 18 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACATGGTCACAGATTCATCGCGCCGCGTCAACAACCCATGTCAACAC |
| Internal bar code: | CGGGTACTACGTGCCAGTTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4570 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGCAGGACGAGGAGGAT |
| Suggested primer 2: | CGTGCACCATTTTCCTGCAA |