Insertion junction: CLIP2.002505_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.002505
Chromosome:chromosome 6
Location:8567875
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g309650 (1 of 1) IPR003200//IPR006626//IPR011050 - Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase-like // Parallel beta-helix repeat // Pectin lyase fold/virulence factor intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCATCCCAGGCTCACCTCCCTTCTCTCTCGCGTCGTTCCACCAGGCCG
Internal bar code:CAGATCCCCCACGTTGTGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:3602
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:CATGCTGACATGCACGCTAC
Suggested primer 2:GATCTACCCGACGAGGCAAG