| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.002518 |
| Chromosome: | chromosome 10 |
| Location: | 53187 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g417750 | (1 of 10) 3.1.1.5 - Lysophospholipase / Phospholipase B | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGCGCATGAAGAATACACTAGAGTGGACGCGATAACACGGTGTGCTG |
| Internal bar code: | GTATGGGAAATTTGAGGCCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 307 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGGAACTCGGTGGGTAG |
| Suggested primer 2: | GGGTATGGGTGGGCATTGAA |