Insertion junction: CLIP2.002529_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.002529
Chromosome:chromosome 3
Location:3172462
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g165000 EFG10,LPA1 (1 of 1) K03596 - GTP-binding protein LepA (lepA); Translation elongation factor EFG/EF2, LepA-related intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCAAGGCTGCCGCAGGTCACGTGACAGCGTGTGAACACCTGGCAGCCG
Internal bar code:TGATCGGTCGATCGGTTATGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:61
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTACTGTACCTGGCTACG
Suggested primer 2:CGAACCCCCTCAGTTTTCCA