Insertion junction: CLIP2.002531_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.002531
Chromosome:chromosome 12
Location:9000823
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g545101 XDH1 (1 of 1) 1.17.1.4 - Xanthine dehydrogenase / Xanthine/NAD(+) oxidoreductase; Xanthine dehydrogenase/oxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAAACTGGTACTCAAACACACGTACACACACGCACCCCCCCCCCCGCCA
Internal bar code:TAAGGTGCAGCGGGAAAGTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:249
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AACTCTGCACTGCCGTAGTC
Suggested primer 2:TCACGGTGTCTCTTTGCTCC