Insertion junction: CLIP2.002531_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.002531
Chromosome:chromosome 12
Location:9000831
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g545101 XDH1 (1 of 1) 1.17.1.4 - Xanthine dehydrogenase / Xanthine/NAD(+) oxidoreductase; Xanthine dehydrogenase/oxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAAACTCGCGCGCACGTCTGCGTGTGCGTGGAAGGGCAGGCGTGCCGAGC
Internal bar code:TGTTGAGAGGGGGGCATACTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:240
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TCACGGTGTCTCTTTGCTCC
Suggested primer 2:AACTCTGCACTGCCGTAGTC