Insertion junction: CLIP2.002551_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.002551
Chromosome:chromosome 10
Location:2794296
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g438650 ADK2 Adenylate kinase; (1 of 1) 2.7.4.14 - UMP/CMP kinase / Uridine monophosphate-cytidine monophosphate phosphotransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGACCAGAAAGGACGGTAAAGAGGTGGCGTGAGATTCACCGTAACTGC
Internal bar code:TCTGGGAGGTTAATGCAAGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2312
LEAP-Seq percent confirming:95.2381
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGTCTTCCCGCGAGCTAG
Suggested primer 2:GCAATACCCACCAGTGTCCA