| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.002560 |
| Chromosome: | chromosome 4 |
| Location: | 3453773 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g227700 | CDS | ||
| Cre04.g227750 | (1 of 1) PF08573 - DNA repair protein endonuclease SAE2/CtIP C-terminus (SAE2) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCACCACCAACTATGTGGGCATGCGGACCGGCGCCGCCATCACTGCG |
| Internal bar code: | GCTGCAATCAGCGCTGTAAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1203 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCAGACATCTACGAGCC |
| Suggested primer 2: | CACTACACCCACCATCCCAC |