| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.002571 |
| Chromosome: | chromosome 16 |
| Location: | 3222458 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g665750 | MAA2,RAA2 | psaA mRNA trans-splicing factor; (1 of 3) 5.4.99.25 - tRNA pseudouridine(55) synthase / tRNA Psi(55) synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATGGACATGAGGCGTAGCAGCCTTCTCCAGTGCGCCATTCCGTCCACA |
| Internal bar code: | CGAGCAAATAGGGCCATAAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2336 |
| LEAP-Seq percent confirming: | 15.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATACTACGAGGAGCGCGTG |
| Suggested primer 2: | AAATGGAGCACTGAGGCGAA |