Insertion junction: CLIP2.002577_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.002577
Chromosome:chromosome 14
Location:503966
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g611250 POC9 Proteome of centriole protein 9; (1 of 2) PTHR12086:SF9 - EF-HAND DOMAIN-CONTAINING PROTEIN 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATACAGGGGTCGGTGTGTGATCGGGTGGGGCCGCAGTCAACCCGAACCC
Internal bar code:GTGTCGACTACTACTATCGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3209
LEAP-Seq percent confirming:97.9167
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTTCAGGGCTACTACCAG
Suggested primer 2:CTGACTTCCAGGAGCAACGT