| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.002594 |
| Chromosome: | chromosome 17 |
| Location: | 4536974 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g731571 | intron | ||
| Cre17.g802093 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAAGTAGACCATTTATGGCACCACATTGGCGCGCGTAGAGCGGGCCGAG |
| Internal bar code: | CGAACGGGACAGGTTACGTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 9813 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 84 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCAGAGGGGGTAGTTTCC |
| Suggested primer 2: | ACAGACAGTGTCTTGGCTCG |