Insertion junction: CLIP2.002597_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.002597
Chromosome:chromosome 10
Location:997873
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g424775 CTP2,PCH1,PAA1 Copper transporting ATPase and Cu chaperone for CTP1; (1 of 1) PTHR24093:SF124 - COPPER-TRANSPORTING ATPASE PAA1, CHLOROPLASTIC CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGCGGCGCTGGCGGCGGCGGACGTGGGCGTGGCCATGGGCGGCGGTGT
Internal bar code:AAACGAGTGTATCTGACTCGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1075
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCTAACAAGCGACGCAAG
Suggested primer 2:TATAATCCAGCCCCCTGCCT