| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.002610 |
| Chromosome: | chromosome 14 |
| Location: | 2294817 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g622550 | (1 of 1) PTHR10953//PTHR10953:SF145 - UBIQUITIN-ACTIVATING ENZYME E1 // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTATGAGTACGCGCTTGTGAACCGCCAGCCGCGCAGGCGCGACGAGC |
| Internal bar code: | ACCCTGAACGAGTCCTCATTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5227 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGCGCAGAAAGGTTGTTG |
| Suggested primer 2: | GTGTTTGAACCCGAACAGCC |