| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.002619 |
| Chromosome: | chromosome 3 |
| Location: | 8798308 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206929 | RSE4,EGY1 | (1 of 1) PTHR31412:SF0 - ZINC METALLOPROTEASE EGY1, CHLOROPLASTIC-RELATED; membrane associated metalloprotease involved in chloroplast development | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGCTGATCAGCACCTCGTCGGTGCCCGCGGTCAGCTGCCAGGCGGAC |
| Internal bar code: | CCATAATTGCCCATTAAAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1118 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAAAGAGACGCTGGAGTT |
| Suggested primer 2: | GCATGGTTAGCATGAGCAGC |