Insertion junction: CLIP2.002631_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.002631
Chromosome:chromosome 3
Location:548622
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145207 CPLD33 Conserved in the Plant Lineage and Diatoms; (1 of 1) PTHR36359:SF1 - RESISTANCE TO PHYTOPHTHORA 1 PROTEIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTATGAAGGAAGCAAGCGGGGCCGATGCAAGTGCGGGCGAAGGCAAC
Internal bar code:AACCTTTGTCTTACGTGAGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1908
LEAP-Seq percent confirming:97.8261
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGCACTCGAGGATCCAGC
Suggested primer 2:ACCTCAATGCCATCCCACAG