Insertion junction: CLIP2.002632_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.002632
Chromosome:chromosome 1
Location:7738324
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g054500 (1 of 1) 1.6.1.2 - NAD(P)(+) transhydrogenase (Re/Si-specific) / Transhydrogenase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGCACGGTGCGTGGCGCGGCTTTCGCCTGGACCCGTTTGGAACGGGCG
Internal bar code:CTGTAACGATCCCTTGACGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1716
LEAP-Seq percent confirming:96.6667
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCACAGTTGTCAACAGCC
Suggested primer 2:TACGGTTTTTCGCTCGTGGA