Insertion junction: CLIP2.002632_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.002632
Chromosome:chromosome 1
Location:7738332
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g054500 (1 of 1) 1.6.1.2 - NAD(P)(+) transhydrogenase (Re/Si-specific) / Transhydrogenase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACCCCACACCCGGGGGCCTGCCCATCCTCCTGTAACTCCACATCCAGA
Internal bar code:CTGTAACGATCCCTTGACGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1713
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:TACGGTTTTTCGCTCGTGGA
Suggested primer 2:CAGCACAGTTGTCAACAGCC