Insertion junction: CLIP2.002633_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.002633
Chromosome:chromosome 1
Location:5397681
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g038050 COP7,HKR3,COP Histidine kinase rhodopsin 3; (1 of 1) PF00072//PF00512//PF01036 - Response regulator receiver domain (Response_reg) // His Kinase A (phospho-acceptor) domain (HisKA) // Bacteriorhodopsin-like protein (Bac_rhodopsin) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCGGTGCCTTCGGCTCGCGCATGCTCGGCGGCTCCACGTGTGTGACGG
Internal bar code:AATACGCCCTACGGTCTGGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1360
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCAGCATCAACAACTTGC
Suggested primer 2:ACCACAACCGTATCCGTCAC