| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.002640 |
| Chromosome: | chromosome 5 |
| Location: | 2410166 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238500 | (1 of 4) K06941 - 23S rRNA (adenine2503-C2)-methyltransferase [EC:2.1.1.192] (rlmN) | 3'UTR | |
| Cre05.g238550 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTCAAGTAGCACAATTGCAGTAATTGAAGAACTGTTGTCCAATAGACT |
| Internal bar code: | GTCAGATGCGCATGTGCCATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4453 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGTGTAGAAGGCAAGGG |
| Suggested primer 2: | CTTGTTGCGGTGAAGTTGGG |